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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Three-Dimensional Microscopy in Microbiology01:28

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Related Experiment Video

Updated: Jun 10, 2026

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Video microscope with submicrometer resolution.

S Mechels, M Young

    Applied Optics
    |August 12, 2010
    PubMed
    Summary
    This summary is machine-generated.

    We developed a video microscope for precise optical fiber geometry measurements. It achieves high accuracy for distance but has minor systematic errors in width measurements.

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    Area of Science:

    • Optical engineering
    • Metrology

    Background:

    • Accurate measurement of optical fiber geometry is crucial for telecommunications and photonics.
    • Existing methods may have limitations in precision or ease of use.

    Purpose of the Study:

    • To construct and evaluate a novel video microscope system.
    • To assess its capability for precise optical fiber geometry measurements.

    Main Methods:

    • A video microscope was built using a frame transfer video camera, specialized optics (40x, 0.65 N.A. objective), and digital electronics.
    • Simple digital algorithms were employed for data analysis.

    Main Results:

    • The system demonstrated precise distance measurements with a random uncertainty of approximately 40 nm across the 150 x 150 micrometer field of view.
    • Width measurements exhibited a systematic error ranging from 0.1 to 0.2 micrometers.

    Conclusions:

    • The developed video microscope is a viable tool for accurate optical fiber geometry analysis.
    • Further refinement may be needed to address systematic errors in width measurements.