Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Humans homozygous for rare or common hypomorphic IL23R variants are prone to tuberculosis.

The Journal of experimental medicine·2026
Same author

Opposing Association of Lung Neutrophils and PD-L1<sup>+</sup> Monocytes in Age-Related Severity of SARS-CoV-2 Infection.

Aging cell·2026
Same author

Clonal expansion of CAR-T cells harboring lentivector integration in the PLAAT4 gene following anti-CD19 CAR T-cell therapy in a context of cutaneous T-cell lymphoma.

Haematologica·2026
Same author

Gastrointestinal stenosis: an underrecognized complication of CARMIL2 deficiency.

Allergologia et immunopathologia·2026
Same author

High risk of hypoxemic COVID-19 pneumonia in myasthenia gravis patients with type I IFN autoantibodies.

medRxiv : the preprint server for health sciences·2026
Same author

Homozygosity for rare or common hypomorphic <i>IL23R</i> variants confers a predisposition to tuberculosis in humans.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 10, 2026

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
12:17

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells

Published on: January 2, 2016

NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.

Vivien Béziat1, Benjamin Descours, Christophe Parizot

  • 1INSERM UMR-S 945, Hôpital Pitié-Salpêtrière, and Université Pierre et Marie Curie (Paris-6), Paris, France.

Plos One
|August 12, 2010
PubMed
Summary
This summary is machine-generated.

Mature human NK cells differentiate from CD56bright to CD56dim subsets, with NKG2A decreasing and KIRs increasing. This process determines NK cell responsiveness and function, guiding potential reclassification of NK cell subsets.

More Related Videos

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
10:24

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Published on: December 3, 2017

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
06:53

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Published on: January 13, 2023

Related Experiment Videos

Last Updated: Jun 10, 2026

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
12:17

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells

Published on: January 2, 2016

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
10:24

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Published on: December 3, 2017

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
06:53

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Published on: January 13, 2023

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Natural Killer (NK) cell terminal differentiation is vital for pathogen response and self-cell discrimination.
  • KIRs and NKG2A are key in NK cell education, influencing competence or hyporesponsiveness, but differentiation events remain debated.

Purpose of the Study:

  • To investigate the terminal differentiation stages of NK cells.
  • To characterize gene expression, surface receptors, and effector functions of differentiating NK cell subsets.
  • To explore the relationship between NKG2A and KIR expression in NK cell functional competence.

Main Methods:

  • Utilized complementary approaches to assess subset kinetics during development.
  • Performed pan-genomic level gene expression profiling.
  • Analyzed distinct surface receptor patterns and effector functions.

Main Results:

  • Supported the hypothesis that CD56dim NK cells arise from CD56bright cells.
  • Demonstrated an inverse correlation between NKG2A and KIR expression.
  • Identified CD56dimNKG2A-KIR+ cells as highly differentiated, responsive to HLA-E+ targets.
  • Observed NKG2A reacquisition correlating with IFN-gamma production post-stimulation.

Conclusions:

  • Findings suggest a reclassification of mature human NK cells into distinct subsets.
  • Proposed a new model where NK cell differentiation involves decreased NKG2A and increased KIR acquisition.
  • Indicated that persistent inhibitory signals during education shape NK cell responsiveness.