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Related Concept Videos

Transcription Factors02:16

Transcription Factors

82.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.9K
Transcription Elongation Factors02:35

Transcription Elongation Factors

14.1K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
14.1K
Transcription Elongation Factors02:35

Transcription Elongation Factors

4.8K
No description available
4.8K
General Transcription Factors01:30

General Transcription Factors

7.1K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.1K
Factors Affecting Protein-Drug Binding: Protein-Related Factors01:20

Factors Affecting Protein-Drug Binding: Protein-Related Factors

576
Drug binding to proteins is a key aspect of pharmacokinetics and can influence a drug's distribution, absorption, and elimination in the body. Several factors, including the drug's physiochemical properties, protein concentration, disease states, and the number of binding sites on the protein, influence this process.
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
576
Transcription01:10

Transcription

157.1K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
157.1K

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Related Experiment Video

Updated: Feb 11, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

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Transcription factor sequestration by polyglutamine proteins.

Tomoyuki Yamanaka, Nobuyuki Nukina

    Methods in Molecular Biology (Clifton, N.J.)
    |August 12, 2010
    PubMed
    Summary

    Polyglutamine diseases like Huntington's disease involve protein aggregates that sequester transcription factors. This chapter details methods to study these interactions in neurons, crucial for understanding disease mechanisms.

    Area of Science:

    • Neuroscience
    • Genetics
    • Molecular Biology

    Background:

    • Polyglutamine diseases, such as Huntington's disease, are characterized by nuclear aggregates of mutant proteins in neurons.
    • These aggregates sequester essential transcription factors, disrupting normal cellular functions.
    • Altered gene expression patterns are observed in polyglutamine disease models, indicating transcriptional dysregulation.

    Purpose of the Study:

    • To introduce methods for examining the interaction between transcription factors and expanded polyglutamine proteins.
    • To describe techniques for assessing the sequestration of transcription factors by polyglutamine aggregates.
    • To provide tools for in vitro and in vivo studies of these molecular interactions.

    Main Methods:

    • In vitro biochemical assays to detect protein-protein interactions.

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    Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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    High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
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  • Cellular models to visualize and quantify aggregate formation and transcription factor sequestration.
  • In vivo studies in animal models to assess these interactions within the neuronal environment.

    • Main Results:

    • Established methods allow for the detection of direct interactions between expanded polyglutamine proteins and transcription factors.
    • Quantifiable sequestration of specific transcription factors by nuclear aggregates has been demonstrated.
    • These interactions are shown to occur both in controlled laboratory settings and within living organisms.

    Conclusions:

    • Transcriptional dysregulation is a key pathological mechanism in polyglutamine diseases.
    • The presented methods are vital for dissecting the molecular basis of these neurodegenerative disorders.
    • Understanding transcription factor sequestration provides potential therapeutic targets for polyglutamine diseases.