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[Chromogenic medium for detection of enterobacteria producing beta-lactamases in North-Eastern Romania]
Cristina Tuchiluş1, Antonia Poiată, D Buiuc
1Universitatea de Medicină şi Farmacie Gr.T. Popa Iaşi, Facultatea de Medicină, Disciplina de Microbiologie.
Unlabelled:
We evaluated the performances and analyzed practicability of an ESBL-detecting chromogenic agar medium (chromID ESBL, bioMerieux) for the screening of ESBL production.
Material And Methods:
We have tested 112 enterobacteria strains, isolated in 2009 year, in North Eastern region of Romania. Each bacterial strain was cultured on a prepared plate chromogenic medium and the results were recorded after incubation at 37 degrees C for 18 to 24 h or, if necessary, for 48 h. We calculated the sensitivities, specificities, and positive and negative predictive values for this medium, using as gold standard ceftzidime or cefotaxime and clavulanate combination disks on Iso-Sensitest agar. K. pneumoniae ATCC 700603 and E. coli ATCC 25922 were used as the positive and negative control for ESBL production, respectively.
Results:
For all tested strains, the chromID ESBL agar showed a high sensitivity (97%), but a low specificity (66%). The positive and negative predictive values were 98% and 57%, respectively. The chromID ESBL had false-positive results for 2 confirmed ESBL-positive strains.
Conclusion:
The very good sensitivity of the chromID ESBL agar ESBL detection represent a convenient method for the recovery and identification of ESBL-producing enterobacteria.
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