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Effect of acute ethanol administration and nutritional status on secretory protein synthesis in isolated rat liver
T Girbés1, J M Ferreras, R Muñoz
1Departamento de Bioquimica, Biologia Molecular y Fisiologia, Facultad de Ciencias, Universidad de Valladolid, 47005 Valladolid, Spain.
Abstract:
Treatment of isolated rat liver cells with 20 mm-ethanol inhibited basal secretory protein synthesis by 30% only when the donors were starved for 48 hr, immediately before they were killed. This inhibition was unaffected by the presence of ethanol in the diet of the donor animals. Independently, d-glucose and l-proline enhanced rates of secretory protein synthesis in a dose-dependent manner but only in cells from 48-hr-fasted donors. This latter stimulation was prevented by the presence of 20 mm-ethanol in the incubation medium. By contrast, up to 100 mm-ethanol did not alter polypeptide synthesis by a post-mitochondrial supernatant from rat liver.
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