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Published on: June 2, 2023
Lack of di-(2-ethylhexyl) phthalate activity in the C3H 10T 1 2 cell transformation system
J H Sanchez1, D J Abernethy, C J Boreiko
1Department of Genetic Toxicology, Chemical Industry Institute of Toxicology, P.O. Box 12137, Research Triangle Park, NC 27709, USA.
Abstract:
The widely used plasticizer and rodent carcinogen di-(2-ethylhexyl) phthalate (DEHP) was examined for activity in the C3H 10T 1 2 murine fibroblast cell transformation system. Treatment with DEHP or its metabolite, mono-(2-ethylhexyl) phthalate, did not produce oncogenic transformation, initiate the process of transformation in cultures treated with a tumour promoter or promote the process of transformation in cultures pretreated with a chemical carcinogen. These findings are consistent with the suggestion that the carcinogenicity of DEHP is mediated by an indirect mechanism and not by covalent interaction of DEHP with DNA.
