Expression of CD200/CD200R regulatory molecules on granulocytes and monocytes is modulated by cardiac surgical
Jan Krejsek1, Martina Kolackova, Jiri Mandak
1Department of Clinical Immunology and Allergy, Charles University in Prague, Faculty of Medicine and University Hospital in Hradec Kralove, Czech Republic. krejsek@fnhk.cz
Insights
Cardiac surgery alters immune responses. CD200 receptor expression increased on granulocytes, while CD200 and CD200 receptor expression decreased on monocytes post-surgery.
Area of Science:
- Immunology
- Cardiovascular Surgery
Background:
- Cardiac surgery triggers inflammatory responses impacting homeostasis.
- Immune regulation involves humoral and cellular mechanisms.
- CD200 and CD200-Receptor (CD200R) are key regulatory molecules.
Purpose of the Study:
- To investigate changes in CD200/CD200R expression on monocytes and granulocytes after cardiac surgery.
- To compare these changes between standard open-heart (OP) and minimally invasive off-pump (MOP) procedures.
Main Methods:
- Flow cytometry was used to quantify CD200 and CD200R expression.
- Monocytes and granulocytes were analyzed from cardiac surgery patients.
Main Results:
- CD200R expression on granulocytes increased post-surgery, significantly higher in OP patients.
- CD200R expression on monocytes decreased post-surgery in both groups.
- CD200 expression on monocytes decreased post-surgery, but increased by day 3 in OP patients.
- CD200 expression on granulocytes was higher in OP patients compared to MOP patients.
Conclusions:
- Cardiac surgery significantly alters CD200/CD200R expression on immune cells.
- CD200R increases on granulocytes, while CD200 and CD200R decrease on monocytes.
- Differences in expression patterns may relate to surgical approach (OP vs. MOP).
Aims:
Cardiac surgical operation is inseparably linked to the induction of an inflammatory response. Both humoral and cellular regulatory mechanisms are operating to maintain body homeostasis. We followed the changes in the expression of CD200/CD200R regulatory molecules on monocytes and granulocyte of cardiac surgical patients operated on using either standard (OP) or modified "mini-invasive" cardiopulmonary bypass (MOP).
Methods:
Expression of CD200/CD200R regulatory molecules was determined by flow cytometry.
Results:
The expression of CD200R on granulocytes was increased after surgery in both groups of patients, but the increase was statistically significant only in OP patients (p<0.01). At this time point, there was a significant difference in CD200R expression on granulocytes when comparing OP to MOP patients, being higher in the former group (p<0.01). The expression of CD200R on monocytes was diminished after surgery and during an early postoperative period in both groups of patients. The expression of CD200 on monocytes was significantly diminished after surgery in both groups (p<0.01). Nonetheless, we observed an increase in CD200 expression in OP patients at the 3rd postoperative day. There was a statistically significantly increased CD200 expression on monocytes of OP patients (p<0.001) at the 3rd postoperative day when we compared OP and MOP groups. The expression of CD200 on granulocytes was significantly higher after surgery and at the 3rd postoperative day in OP when compared to MOP patients.
Conclusions:
CD200R expression on granulocytes was significantly increased, while CD200 and CD200R expression on monocytes was decreased after cardiac surgery.
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