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Related Concept Videos

Integrins01:10

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Activation of Integrins01:15

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved in a...
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved in a...

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Related Experiment Video

Updated: Jun 10, 2026

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

RET-mediated cell adhesion and migration require multiple integrin subunits.

Jessica G Cockburn1, Douglas S Richardson, Taranjit S Gujral

  • 1Division of Cancer Biology and Genetics, Cancer Research Institute, and Department of Pathology and Molecular Medicine at Queen's University, Kingston, Ontario, Canada.

The Journal of Clinical Endocrinology and Metabolism
|August 13, 2010
PubMed
Summary
This summary is machine-generated.

RET receptor tyrosine kinase (RTK) signaling activates multiple integrin subunits, including beta-1 (ITGB1) and beta-3 (ITGB3), to drive cancer cell migration and invasion. This highlights integrins as key players in RET-mediated diseases.

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Last Updated: Jun 10, 2026

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

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07:55

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Published on: March 8, 2017

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers
05:43

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

Published on: November 8, 2024

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Signaling

Background:

  • The RET receptor tyrosine kinase (RTK) is implicated in neuroendocrine cancers, where aberrant cell migration is a hallmark.
  • Integrins are critical regulators of cell migration, a process often dysregulated in cancer.

Purpose of the Study:

  • To investigate the role of integrins in RET-mediated cell migration using neural-derived (SH-SY5Y) and thyroid cancer (TPC-1) cell lines.
  • To determine if multiple integrin subunits are involved in RET-driven cell migration.

Main Methods:

  • Assessed integrin expression and activation via cell adhesion and wound-healing assays.
  • Examined integrin activity by analyzing focal adhesion formation using coimmunoprecipitation and immunofluorescence.
  • Evaluated RET-mediated cell migration and invasion in vitro and in vivo mouse tumor xenograft models.

Main Results:

  • Beta-1 integrin (ITGB1) is expressed in both cell lines and mediates cell adhesion to ITGB1-associated matrices.
  • RET activation leads to ITGB1 activation, which is essential for RET-induced cell adhesion and migration.
  • Beta-3 integrin (ITGB3) also contributes to RET-mediated cell adhesion and migration, with its expression correlating with invasion in vivo.

Conclusions:

  • Multiple integrin subunits (ITGB1 and ITGB3) are downstream effectors of RET signaling in cell migration and adhesion.
  • Coordinated signaling through RET and integrins is crucial for cancer cell interactions with the microenvironment during invasion and progression.