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Related Experiment Video

Updated: Jun 10, 2026

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

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Published on: September 20, 2017

Comprehensive microRNA expression profiling of the hematopoietic hierarchy.

O I Petriv¹, F Kuchenbauer, A D Delaney

¹Center for High-Throughput Biology, Department of Physics and Astronomy, and Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada V6T 1Z4.

Proceedings of the National Academy of Sciences of the United States of America

|August 13, 2010

Summary

MicroRNAs (miRNAs) orchestrate hematopoietic stem cell differentiation. This study mapped miRNA expression across hematopoietic hierarchy, revealing lineage relationships and functional similarity for improved cell compartment analysis.

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Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

Hematology
Molecular Biology
Genomics

Background:

The hematopoietic system generates diverse cell types from common stem cells.
MicroRNAs (miRNAs) are key regulators of hematopoietic differentiation.
Understanding miRNA expression is crucial for deciphering hematopoietic hierarchy.

Purpose of the Study:

To develop and apply a high-throughput microfluidic real-time quantitative PCR (RT-qPCR) method.
To generate global miRNA expression profiles for 27 distinct mouse hematopoietic cell populations.
To map the miRNA landscape across the hematopoietic hierarchy, including rare stem and progenitor cells.

Main Methods:

Development of a high-throughput microfluidic RT-qPCR platform.
Isolation of 27 phenotypically distinct hematopoietic cell populations from adult mouse tissues.
Performance of 80,000 RT-qPCR assays to profile miRNA expression.

Main Results:

miRNA expression profiles enabled direct inference of cell lineage relationships and functional similarity.
Multipotent progenitors and stem cells exhibited closely related miRNA expression patterns.
A significant miRNA reprogramming occurred upon lineage potential restriction.

Conclusions:

miRNA profiling is a powerful tool for understanding hematopoietic hierarchy and cell relationships.
Single-cell miRNA analysis highlights tight regulation and potential for assessing compartment heterogeneity.
This approach provides a comprehensive view of miRNA's role in hematopoietic differentiation.