Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interaction between nephrocalcin and calcium oxalate monohydrate: a structural study.

S Deganello1

  • 1Nephrology Program, University of Chicago, IL 60637.

Calcified Tissue International
|June 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sialic acid-containing glycoproteins on renal cells determine nucleation of calcium oxalate dihydrate crystals.

Kidney international·2001
Same author

Nucleation, adhesion, and internalization of calcium-containing urinary crystals by renal cells.

Journal of the American Society of Nephrology : JASN·1999
Same author

Cell-crystal interactions and kidney stone formation.

Nephron·1999
Same author

Direct nucleation of calcium oxalate dihydrate crystals onto the surface of living renal epithelial cells in culture.

Kidney international·1998
Same author

Face-selective adhesion of calcium oxalate dihydrate crystals to renal epithelial cells.

Calcified tissue international·1996
Same author

The interaction between nephrocalcin and Tamm-Horsfall proteins with calcium oxalate dihydrate.

Scanning microscopy·1993

Nephrocalcin protein significantly alters calcium oxalate monohydrate crystal formation, affecting size, structure, and growth patterns. Higher concentrations lead to severe growth inhibition and crystallite aggregation.

Area of Science:

  • Crystallography
  • Biomineralization
  • Materials Science

Background:

  • Calcium oxalate monohydrate (COM) crystals are implicated in kidney stone formation.
  • Understanding protein interactions with COM is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To investigate the impact of nephrocalcin protein on the growth and structure of calcium oxalate monohydrate crystals.
  • To elucidate the mechanism by which nephrocalcin influences COM crystallization.

Main Methods:

  • Crystallization of calcium oxalate monohydrate from gels in the presence of varying concentrations of nephrocalcin.
  • Analysis of crystal habit, size, and structure using diffraction patterns.

Main Results:

Related Experiment Videos

  • Nephrocalcin profoundly affects COM crystal habit, size, and structure.
  • At 26.2 x 10(-7) M, COM undergoes a phase change, with reduced crystal size and diffraction pattern resolution.
  • At higher concentrations (52.5-200 x 10(-7) M), severe growth inhibition, long-range disorder, and aggregation of small crystallites are observed.

    • Conclusions:

    • Nephrocalcin disrupts the organization of oxalate groups and water molecules, hindering the juxtaposition of (101) layers.
    • The stereospecific adsorption of nephrocalcin onto (101) planes modulates its inhibitory effect on COM growth.