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GTPase regulator associated with the focal adhesion kinase (GRAF) transcript was down-regulated in patients with

Zhen Qian1, Jun Qian, Jiang Lin

  • 1Department of Hematology, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, China. qianjun0007@hotmail.com

Journal of Experimental & Clinical Cancer Research : CR
|August 14, 2010
PubMed
Abstract

Insights

The GTPase regulator associated with focal adhesion kinase (GRAF) transcript is significantly decreased in myeloid malignancies, including acute myeloid leukemia, myelodysplastic syndrome, and chronic myeloid leukemia. This suggests GRAF may play a role in these blood cancers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Hematology

Background:

  • GTPase regulator associated with focal adhesion kinase (GRAF) is a potential tumor suppressor gene.
  • GRAF gene inactivation is observed in hematopoietic malignancies.
  • GRAF gene expression levels in leukemia have not been previously studied.

Purpose of the Study:

  • To investigate the expression level of the GRAF gene in patients with myeloid malignancies.
  • To analyze GRAF gene expression in acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and chronic myeloid leukemia (CML).

Main Methods:

  • Quantitative real-time PCR (RQ-PCR) was used to determine GRAF transcript levels in 94 patients.
  • Clinical and laboratory data were collected and analyzed.

Main Results:

  • GRAF transcript levels were significantly decreased in AML, MDS, and CML compared to controls.
  • No significant difference in GRAF transcript levels was found among AML FAB subtypes.
  • GRAF transcript levels decreased as CML progressed.
  • MDS cases with 5q deletion showed lower GRAF transcript levels.

Conclusions:

  • The GRAF transcript is demonstrably decreased in myeloid malignancies.
  • GRAF downregulation may be associated with the development or progression of these hematologic disorders.

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