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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Updated: Jun 10, 2026

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
09:40

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Published on: February 13, 2012

Conditional RNAi in mice.

Aljoscha Kleinhammer1, Jan Deussing, Wolfgang Wurst

  • 1Institute for Developmental Genetics, Helmholtz Center Munich - German Research Center for Environmental Health, Ingolstädter Landstr. 1, 85764 Neuherberg/Munich, Germany. aljoscha.kleinhammer@helmholtz-muenchen.de

Methods (San Diego, Calif.)
|August 14, 2010
PubMed
Summary
This summary is machine-generated.

We developed a novel RNA interference (RNAi) strategy for rapid gene knockdown in mice. This method enables precise temporal and tissue-specific gene silencing for efficient in vivo functional studies.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • RNA interference (RNAi) is a standard method for gene function analysis in cell cultures.
  • Short hairpin (sh) RNAs offer a rapid alternative to traditional knockout methods for gene knockdown in mice.

Purpose of the Study:

  • To establish a versatile strategy for complete or conditional gene knockdown in mice.
  • To enable time- and tissue-specific gene silencing for in vivo studies.
  • To facilitate rapid and reproducible generation of knockdown mouse models.

Main Methods:

  • Utilized the Cre/loxP system for inducible RNAi activation.
  • Employed doxycycline-controlled shRNA expression vectors for conditional gene silencing.
  • Integrated single-copy RNAi constructs into the Rosa26 locus of ES cells via recombinase-mediated cassette exchange.
  • Generated germline transmission through chimeric mice and subsequent crossing with Cre transgenic strains.

Main Results:

  • Successfully developed a method for stable shRNA expression integrated into the mouse genome.
  • Demonstrated the ability to achieve conditional gene knockdown using Cre/loxP or doxycycline systems.
  • Facilitated the production of reproducible knockdown mouse models for phenotypic analysis.

Conclusions:

  • The developed strategy provides an efficient and reproducible approach for generating knockdown mice.
  • This method significantly accelerates the assessment of gene function in vivo.
  • Site-specific insertion ensures consistent gene silencing and facilitates genetic studies.