Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...

Nonsense-mediated mRNA Decay

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The microprotein SMIM26 connects metabolite transporters of the outer and inner mitochondrial membranes and is essential for respiratory chain function.

Genes & development·2026

Same author

In vivo AGO-APP for cell-type- and compartment-specific miRNA profiling in the mouse brain.

Cell reports methods·2025

Same author

Pachytene piRNAs define a conserved program of meiotic gene regulation: Pachytene piRNAs directly regulate select mRNAs by and RNAi-like mechanism, establishing a regulatory paradigm of mammalian meiosis.

Research square·2025

Same author

Pachytene piRNAs define a conserved program of meiotic gene regulation: Pachytene piRNAs directly regulate select mRNAs by and RNAi-like mechanism, establishing a regulatory paradigm of mammalian meiosis.

bioRxiv : the preprint server for biology·2025

Same author

The liver talks back: NPY orchestrates attraction of cancer cells and CHK2-dependent clonogenicity in the metastatic niche.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

YTHDF proteins and m<sup>6</sup>A-RNA clients undergo autophagic turnover during contact inhibition.

Cell reports·2025

Same journal

ChAHP silences SINE retrotransposons by inhibiting TFIIIB recruitment.

Molecular cell·2026

Same journal

Remodeling activity of ChAHP restricts transcription factor access to chromatin.

Molecular cell·2026

Same journal

Cryo-EM structure of soluble VPS13C suggests its regulation by a conformational switch and by calmodulin.

Molecular cell·2026

Same journal

HDAC5 depletion promotes hyper-acetylation of FOXA1 and potentiates HIF1α transcriptional activation in pancreatic cancer.

Molecular cell·2026

Same journal

Thyroid cancer-associated EZH1 Q571R mutation drives chromatin compaction and H3K27me3 invasion into active chromatin.

Molecular cell·2026

Same journal

Genome-wide rotational and translational phasing of nucleosomes with human transcription factors.

Molecular cell·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 10, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

SMADs stimulate miRNA processing.

Thomas Treiber¹, Gunter Meister

¹Universität Regensburg, Regensburg, Germany.

|August 14, 2010

Summary

This summary is machine-generated.

SMAD proteins control the creation of specific human microRNAs (miRNAs), which are key gene expression regulators. This study reveals the precise mechanisms behind this crucial regulatory process.

More Related Videos

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Related Experiment Videos

Last Updated: Jun 10, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

MicroRNAs (miRNAs) are critical regulators of gene expression, influencing numerous cellular processes.
The precise regulation of miRNA biogenesis is essential for maintaining cellular homeostasis and preventing disease.
Understanding the factors that control miRNA production is a key area in molecular biology.

Discussion:

This study investigates the role of SMAD proteins in the biogenesis of a specific subset of human miRNAs.
The research provides novel mechanistic insights into how SMAD proteins interact with and influence miRNA production pathways.
The findings highlight a direct link between SMAD signaling and the regulation of specific miRNA expression.

Key Insights:

SMAD proteins directly regulate the biogenesis of a defined group of human microRNAs.
The study elucidates the molecular mechanisms by which SMAD proteins exert their regulatory control over miRNA production.
This work deepens our understanding of gene expression control at the post-transcriptional level.

Outlook:

Further research can explore the broader implications of SMAD-mediated miRNA regulation in various biological contexts and diseases.
Investigating other signaling pathways that intersect with SMAD proteins in miRNA biogenesis could reveal new regulatory networks.
This work may pave the way for therapeutic strategies targeting miRNA biogenesis through SMAD protein modulation.