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Sequence and phylogenetic analysis of the non-structural 3A and 3B protein-coding regions of foot-and-mouth disease

Saber Jelokhani-Niaraki1, Majid Esmaelizad, Morteza Daliri

  • 1Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

Journal of Veterinary Science
|August 14, 2010
PubMed

Insights

The highly virulent A Iran 05 foot-and-mouth disease virus (FMDV) subtype shows close genetic links to viruses from Turkey and Pakistan. Molecular analysis reveals specific amino acid substitutions in the 3A and 3B coding regions.

Area of Science:

  • Veterinary Virology
  • Molecular Epidemiology

Background:

  • Foot-and-mouth disease virus (FMDV) poses a significant threat to livestock globally.
  • The A Iran 05 subtype, identified in Iran in 2005, exhibits high virulence.

Purpose of the Study:

  • To conduct molecular and phylogenetic analysis of the 3A and 3B coding regions of the A Iran 05 FMDV field isolate.
  • To determine the genetic relatedness of the A Iran 05 isolate to other Type A FMDV strains.

Main Methods:

  • Comparative analysis of nucleotide and predicted amino acid sequences of the 3AB gene region.
  • Phylogenetic tree construction using 672 bp 3AB gene sequences from 20 Type A FMDV isolates.

Main Results:

  • The A Iran 05 isolate clustered within lineage A, closely related to FMDV strains from Turkey and Pakistan (2005-2006).
  • Phylogenetic analysis of 3AB sequences identified five distinct lineages among Type A FMDV.
  • A Iran 05 exhibited three amino acid substitutions in the 3A and 3B coding regions compared to related isolates.
  • Nucleotide identity between A Iran 05 and other lineage A isolates was high, estimated at 98%.

Conclusions:

  • The A Iran 05 FMDV subtype shares significant genetic similarity with contemporary strains from neighboring regions.
  • Molecular characterization of the 3AB region provides insights into the evolutionary dynamics of Type A FMDV.

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