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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 10, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Fluorescent angular scattering emissions from dye-filled fibers.

D Abromson, W S Bickel

    Applied Optics
    |August 14, 2010
    PubMed
    Summary

    Backscattered fluorescent emissions increase in laser dye-filled fibers as core diameter shrinks below 23 micrometers. This finding is crucial for developing advanced fiber optic sensors and laser applications.

    Area of Science:

    • Optics and Photonics
    • Materials Science

    Background:

    • Fiber optics are utilized in various sensing and laser applications.
    • Understanding light scattering phenomena in small-core fibers is essential for optimizing device performance.

    Purpose of the Study:

    • To investigate the effect of core diameter on fluorescent angular scattering in laser dye-filled hollow-core quartz fibers.
    • To compare fluorescent scattering with elastic scattering of incident radiation.

    Main Methods:

    • Measurement of fluorescent angular scattering from Coumarin 7 dye-filled hollow-core quartz fibers.
    • Varying fiber core diameters below 23 micrometers.
    • Comparison with elastic scattered incident radiation at 442 nm.

    Main Results:

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    • Fluorescent angular scattering intensity increases as the fiber core diameter decreases below 23 micrometers.
    • Distinct scattering patterns were observed at different fluorescent wavelengths.
    • Differences in scattering were noted between fluorescent and elastic scattering.

    Conclusions:

    • Fiber core diameter significantly influences backscattered fluorescent emissions.
    • The observed phenomenon has implications for the design of miniaturized fiber optic devices.
    • Further research can explore different laser dyes and fiber materials.