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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: Jun 10, 2026

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

Rainbow-enhanced forward and backward glory scattering.

D S Langley, M J Morrell

    Applied Optics
    |August 14, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Rainbow glories significantly enhance light scattering when a sphere

    More Related Videos

    Scanning Light Scattering Profiler (SLPS) Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses
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    Scanning Light Scattering Profiler (SLPS) Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

    Published on: June 6, 2017

    Measuring the Behavioral Effects of Intraocular Scatter
    05:10

    Measuring the Behavioral Effects of Intraocular Scatter

    Published on: February 18, 2021

    Related Experiment Videos

    Last Updated: Jun 10, 2026

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    Scanning Light Scattering Profiler (SLPS) Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses
    06:55

    Scanning Light Scattering Profiler (SLPS) Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

    Published on: June 6, 2017

    Measuring the Behavioral Effects of Intraocular Scatter
    05:10

    Measuring the Behavioral Effects of Intraocular Scatter

    Published on: February 18, 2021

    Area of Science:

    • Optics
    • Scattering theory
    • Physical optics

    Background:

    • Glory scattering is an optical phenomenon observed in light scattering by spherical particles.
    • Normal glory scattering exhibits irradiance proportional to the size parameter x.
    • Specific refractive indices (m) can lead to exceptionally strong glory scattering, known as rainbow glories.

    Purpose of the Study:

    • To investigate the phenomenon of rainbow glories in light scattering.
    • To determine the refractive index values that cause enhanced glory scattering.
    • To model and experimentally verify the scattering characteristics of rainbow glories.

    Main Methods:

    • Determining refractive index values from ray path geometry.
    • Developing a physical-optics model for scattering.
    • Performing Mie theory computations.
    • Conducting experiments with single glass spheres in liquids.

    Main Results:

    • Identified specific refractive index values for rainbow glories.
    • A physical-optics model predicts an a(2)x(4/3) dependence for scattered irradiance in rainbow glories.
    • Mie theory computations confirm rainbow glories and the x(4/3) irradiance factor.
    • Experiments validate the predicted cross-polarized scattering and its dependence on refractive index.

    Conclusions:

    • Rainbow glories represent a significant enhancement of glory scattering.
    • The scattering intensity in rainbow glories follows an a(2)x(4/3) dependency.
    • Experimental results align with theoretical predictions, confirming the role of refractive index in rainbow glories.