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Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
Published on: July 30, 2016
[Effects of exogenous melatonin on active oxygen metabolism of cucumber seedlings under high temperature stress]
Xiang-dong Xu1, Yan Sun, Bo Sun
1College of Horticulture, Northwest A & F University, Yangling 712100, Shannxi, China. xuxd05160202@163.com
Abstract:
Taking the seedlings of cucumber cultivar 'Jinchun 4' as test material, this paper studied the effects of foliar application of exogenous melatonin (MT) on the active oxygen metabolism of the seedlings under high temperature stress. Under the stress, the exogenous MT could significantly decrease the leaf superoxide radical (O2-*) production rate, hydrogen peroxide (H2O2) content, cell membrane permeability (electrolyte leakage), and malonaldehyde (MDA) content, but increase the activities of superoxide radical (SOD), peroxidase (POD), and catalase (CAT), and the contents of ascorbic acid (AsA), glutathione (GSH), and soluble proteins, illustrating that pretreatment with MT could inhibit the reactive oxygen species (ROS) production, raise the antioxidative enzyme activities and antioxidant contents, and decrease the membrane lipid peroxidation and electrolyte leakage to protect the integrity of lipid membranes, being able to alleviate the damage of high temperature stress to the seedlings.
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