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Che-1 promotes tumor cell survival by sustaining mutant p53 transcription and inhibiting DNA damage response
Tiziana Bruno1, Agata Desantis, Gianluca Bossi
1Department of Therapeutic Programs Development, Regina Elena Cancer Institute, Rome, Italy.
Abstract:
Che-1 is a RNA polymerase II binding protein involved in the regulation of gene transcription and, in response to DNA damage, promotes p53 transcription. In this study, we investigated whether Che-1 regulates mutant p53 expression. We found that Che-1 is required for sustaining mutant p53 expression in several cancer cell lines, and that Che-1 depletion by siRNA induces apoptosis both in vitro and in vivo. Notably, loss of Che-1 activates DNA damage checkpoint response and induces transactivation of p73. Therefore, these findings underline the important role that Che-1 has in survival of cells expressing mutant p53.
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