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Extracellular Vesicle Tissue Factor Activity Assay
03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

Plasma levels of microparticle-associated tissue factor activity in patients with clinically suspected pulmonary

P Garcia Rodriguez1, H C J Eikenboom, M E T Tesselaar

  • 1Department of Clinical Oncology, Leiden University Medical Center, Leiden, The Netherlands.

Thrombosis Research
|August 17, 2010
PubMed
Abstract

Insights

Plasma microparticle tissue factor (TF) activity was measured in patients with suspected pulmonary embolism (PE). Elevated MP-TF activity was observed in patients with suspected PE compared to controls, but not specifically in those with confirmed PE.

Area of Science:

  • Hematology
  • Oncology
  • Cardiovascular Medicine

Background:

  • Microparticles (MPs) carrying active tissue factor (TF) are found in cancer patients, especially those with deep vein thrombosis (DVT) or pulmonary embolism (PE).
  • Experimental studies indicate circulating TF-carrying MPs contribute to thrombus formation.

Purpose of the Study:

  • To investigate if unselected patients with acute confirmed PE exhibit elevated TF activity in the MP fraction (MP-TF activity).

Main Methods:

  • Plasma MP-TF activity was assessed in 159 patients with clinically suspected PE and 48 healthy controls.
  • Acute PE diagnosis was confirmed in 54 patients and excluded in 105 patients.

Main Results:

  • Median MP-TF activity was higher in patients with suspected PE (72 fM Xa/min) than in healthy controls (66 fM Xa/min; P<0.05).
  • No significant difference in MP-TF activity was found between confirmed PE patients (84.5 fM Xa/min) and non-PE patients (72 fM Xa/min; P=0.169).
  • Exploratory analysis revealed higher MP-TF activity in suspected PE patients with active cancer (137 fM Xa/min) and cardiovascular disease (131.5 fM Xa/min).

Conclusions:

  • Plasma MP-TF activity was not associated with confirmed PE in patients presenting with clinically suspected PE.
  • Elevated MP-TF activity in suspected PE patients may be linked to co-existing conditions like cancer or cardiovascular disease.

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