Related Experiment Video

Updated: Jun 10, 2026

Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments
06:16

Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments

Published on: May 9, 2019

Time course of vitamin C distribution and absorption after oral administration in SMP30/GNL knockout mice

Mizuki Iwama1, Kentaro Shimokado, Naoki Maruyama

  • 1Department of Geriatrics and Vascular Medicine, Tokyo Medical and Dental University, Tokyo, Japan.

Nutrition (Burbank, Los Angeles County, Calif.)
|August 17, 2010
PubMed
Abstract

Insights

Vitamin C (VC) distribution varies significantly across tissues in mice unable to synthesize it. This study highlights differing VC retention and absorption rates in various organs, using a novel knockout mouse model.

Area of Science:

  • Biochemistry
  • Physiology
  • Animal Models

Background:

  • Vitamin C (VC) is crucial for metabolic and antioxidant functions.
  • Senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice cannot synthesize VC endogenously.
  • Understanding VC tissue distribution is vital for its physiological role.

Purpose of the Study:

  • To investigate the tissue-specific movement and distribution of vitamin C (VC).
  • To utilize SMP30/GNL KO mice as a model for studying VC metabolism and transport.
  • To compare VC retention in different tissues under sufficient and deficient conditions.

Main Methods:

  • Utilized SMP30/GNL KO mice, divided into VC-sufficient and VC-deficient groups.
  • Administered controlled doses of VC in drinking water for one month.
  • Analyzed VC content across 20 different tissues and tracked temporal distribution post-oral administration.

Main Results:

  • VC-deficient mice retained only 13.7% of VC compared to sufficient mice.
  • Tissues with high VC retention (>20%) included cerebellum, white fat, testes, eyeballs, and pancreas.
  • Kidneys and heart showed very low VC retention (<5%).
  • Peak VC levels in liver and kidney occurred at 3 hours, while other tissues like cerebellum and testes peaked at 12 hours post-administration.

Conclusions:

  • Exogenous VC exhibits distinct tissue distribution rates in VC-deficient mice.
  • SMP30/GNL KO mice serve as a valuable model for in vivo VC research.
  • Tissue-specific differences in VC uptake and retention are significant.

More Related Videos

Oral Gavage in Neonatal Mouse Pups and Functional Assessment of Gut Barrier Integrity Using Ussing Chambers07:18

Oral Gavage in Neonatal Mouse Pups and Functional Assessment of Gut Barrier Integrity Using Ussing Chambers

Published on: January 9, 2026

Use of Micropipette-Guided Drug Administration as an Alternative Method to Oral Gavage in Rodent Models02:44

Use of Micropipette-Guided Drug Administration as an Alternative Method to Oral Gavage in Rodent Models

Published on: July 26, 2024

Related Concept Videos

One-Compartment Open Model for IV Bolus Administration: Estimation of Elimination Rate Constant, Half-Life and Volume of Distribution01:09

One-Compartment Open Model for IV Bolus Administration: Estimation of Elimination Rate Constant, Half-Life and Volume of Distribution

The one-compartment open model is a simplified approach used in pharmacokinetics to understand the distribution and elimination of a drug administered through an intravenous bolus. This model assumes rapid drug dispersal throughout the body and elimination using a first-order process. Key pharmacokinetic parameters, such as the elimination rate constant (k), half-life (t1/2), and the apparent volume of distribution (Vd), can be estimated from this model. The elimination rate is calculated from...
Bioavailability: Influencing Factors01:22

Bioavailability: Influencing Factors

Bioavailability refers to the extent and rate at which a drug reaches systemic circulation in its active form. Extent refers to the amount of the drug that makes it into circulation, while rate is the speed at which it enters circulation. It is influenced by several factors critical for optimizing drug formulations, dosing regimens, and therapeutic outcomes.Physicochemical properties of drugs and formulationsThe solubility, stability, and dissolution rate of a drug significantly impact its...
Bioavailability: Overview01:17

Bioavailability: Overview

Bioavailability refers to the proportion of an administered drug that reaches the systemic circulation in its active, unaltered form. It is a crucial pharmacokinetic parameter that determines the effectiveness of a drug in achieving its intended therapeutic outcomes. The route of administration significantly influences bioavailability, with intravenous administration achieving 100% bioavailability as the drug directly enters the bloodstream. In contrast, oral administration often results in...
Bioavailability: Overview01:13

Bioavailability: Overview

Bioavailability refers to the proportion of an unaltered drug that, after administration, enters the systemic circulation and can be distributed to the desired action site. Factors such as gastrointestinal (GI) absorption and liver biotransformation influence the bioavailability of a drug when it is administered orally. When a drug is administered intravenously, it enters the systemic circulation directly; by definition, its bioavailability is assumed to be 100%. The bioavailability of an...
Time Course of Drug Effect01:14

Time Course of Drug Effect

The progression of a drug's impact can be analyzed by examining both the concentration-time course and the effect-time course. The concentration-time course is determined by the drug's half-life and is influenced by factors such as its pharmacokinetics, including absorption, distribution, metabolism, and elimination. The effect of the drug is often related to its concentration in the plasma and is calculated using the maximum drug effect and the plasma concentration that generates 50 percent of...
Bioavailability Study Design: Single Versus Multiple Dose Studies01:11

Bioavailability Study Design: Single Versus Multiple Dose Studies

Bioavailability studies are essential for understanding how a drug is absorbed, distributed, metabolized, and excreted in the body. These studies assess the extent and rate at which the active pharmaceutical agent becomes available at the site of action. The design of bioavailability studies can involve single-dose or multiple-dose regimens, each with distinct advantages and limitations.Single-dose studies are the preferred approach due to their simplicity and reduced drug exposure for...