Strip-based amperometric detection of myeloperoxidase

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensor Technology

Background:

• Myeloperoxidase (MPO) is a key biomarker for acute cardiac events.
• Conventional MPO immunoassays can be time-consuming and costly.
• There is a need for rapid, sensitive, and cost-effective MPO detection methods.

Purpose of the Study:

• To develop a screen-printed strip-based amperometric biosensor for MPO determination.
• To achieve high-sensitivity quantification of physiological MPO levels.
• To evaluate the biosensor's performance in human serum and its resistance to interference.

Main Methods:

• Utilized screen-printed electrodes (SPE) with 3,3',5,5'-tetramethylbenzidine (TMB) as a redox mediator.
• Performed multivariate parameter optimization for the biosensor.
• Tested the biosensor in acetate buffer (pH 4.5) and human serum.

Main Results:

• The biosensor detected physiological MPO levels ranging from 3 to 18 U/L with high sensitivity.
• Investigated and assessed potential interference from common serum electroactive compounds and other peroxidase enzymes.
• Demonstrated successful MPO quantification in both buffer and human serum samples.

Conclusions:

• The developed amperometric biosensor provides a simpler, faster, and more cost-effective alternative to traditional MPO immunoassays.
• This technology holds promise for advancing point-of-care testing for acute cardiac events.
• The biosensor's ability to detect MPO in human serum highlights its clinical applicability.