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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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RecA-independent single-stranded DNA oligonucleotide-mediated mutagenesis

Kenan C Murphy1, Martin G Marinus

  • 1Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655, USA.

F1000 Biology Reports
|August 17, 2010
PubMed
Summary

Bacteriophage lambda’s Beta protein enhances oligonucleotide-mediated mutagenesis in bacteria by protecting DNA from degradation. This single-stranded annealing protein (SSAP) facilitates genetic modifications like insertions, deletions, and substitutions.

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