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Updated: Jun 10, 2026

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
Published on: December 26, 2020
A genetically encoded photocaged Nepsilon-methyl-L-lysine
Yane-Shih Wang1, Bo Wu, Zhiyong Wang
1Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
Abstract:
A photocaged N(epsilon)-methyl-L-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover N(epsilon)-methyl-L-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.
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