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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
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Detection of enzyme activities based on the synthesis of gold nanoparticles in HEPES buffer

Takeshi Serizawa1, Yu Hirai, Mamoru Aizawa

  • 1Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan. t-serizawa@bionano.rcast.u-tokyo.ac.jp

Molecular Biosystems
|August 17, 2010
PubMed

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Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.