Extraction & assay of fibronectin from stored plasma

Fibronectin was extracted from 20 units of 3 days old plasma by the heparin cold precipitation technique using 15 or 30 units of sodium heparin per ml of plasma. Immunoelectrophoresis of the extracted fibronectin showed it to be 131.37 +/- 24.73 times concentrated over fibronectin levels in standard plasma, with a mean recovery of 73.99 +/- 18.40 per cent. Corresponding figures by ELISA were 118.95 +/- 27.99 (concentration) and 73.99 +/- 18.40 (per cent).