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Updated: Jun 10, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Tracer-based metabolomics: concepts and practices.

W-N Paul Lee¹, Paulin N Wahjudi, Jun Xu

¹UCLA Center of Excellence for Pancreatic Diseases, Los Angeles Biomedical Research Institute, 1124 West Carson Torrance, CA 90502, USA. lee@labiomed.org

Clinical Biochemistry

|August 18, 2010

Summary

This summary is machine-generated.

Tracer-based metabolomics integrates tracer methods, "-omics" technologies, and metabolic network modeling. This review details its evolution and applications in understanding cellular responses to drugs and nutrients.

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Area of Science:

Systems biology
Metabolic network modeling
Isotope tracing

Background:

Metabolomics and metabonomics are common, but tracer-based metabolomics offers a targeted, model-driven approach.
Tracer-based metabolomics is complex and less understood than other '-omics' fields.
It combines tracer methodology, high-throughput '-omics' technologies, and constraint-based modeling.

Purpose of the Study:

To review the development of tracer-based metabolomics.
To explain the integration of experimental methods and constraint-based modeling.
To illustrate applications in cellular response studies.

Main Methods:

Tracing metabolic pathways using radioactive and stable isotopes.
Isotopomer analysis via mass spectrometry.
Constraint-based modeling of metabolic networks and flux analysis.

Main Results:

Tracer-based metabolomics has evolved significantly from early isotope studies.
Stable isotopes and mass spectrometry enhance metabolic flux analysis.
Constraint-based modeling provides a theoretical framework for network analysis.

Conclusions:

Tracer-based metabolomics is a powerful, albeit complex, systems biology tool.
It enables detailed investigation of cellular metabolism.
Applications include understanding responses to pharmacological agents and nutrient changes.