Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in human tear protein levels with progressively increasing stimulus.

R J Fullard1, D L Tucker

  • 1University of Alabama, Department of Physiological Optics, Birmingham 35294.

Investigative Ophthalmology & Visual Science
|July 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dark Energy Survey Year 1 Results: Cosmological Constraints from Cluster Abundances, Weak Lensing, and Galaxy Correlations.

Physical review letters·2021
Same author

Constraints on Dark Matter Properties from Observations of Milky Way Satellite Galaxies.

Physical review letters·2021
Same author

Disseminated Beauveria bassiana infection in a patient with acute lymphoblastic leukemia.

Journal of clinical microbiology·2004
Same author

DNA array analysis in a Microsoft Windows environment.

BioTechniques·2002
Same author

The manganese stabilizing protein (MSP) and the control of O2 evolution in the unicellular, diazotrophic cyanobacterium, Cyanothece sp. ATCC 51142.

Biochimica et biophysica acta·2001
Same author

Analysis of chlorophyll-protein complexes from the cyanobacterium Cyanothece sp. ATCC 51142 by non-denaturing gel electrophoresis.

Biochimica et biophysica acta·2000

Tear protein concentrations vary significantly with stimulation levels. Nonstimulated tears have higher levels of many proteins, and simply classifying tears as stimulated or nonstimulated is insufficient to capture these changes.

Area of Science:

  • Ophthalmology
  • Biochemistry
  • Immunology

Background:

  • Tear film composition is crucial for ocular surface health.
  • Understanding tear protein dynamics under varying stimulation is essential for diagnosing and managing ocular conditions.

Purpose of the Study:

  • To quantify the concentration changes of 13 key proteins in human tears across a range of flow rates.
  • To investigate the impact of noninvasive tear collection methods on protein analysis.

Main Methods:

  • Collected six atraumatic tear samples from ten subjects at increasing flow rates (0.5 to >50 microliters/min).
  • Fractionated tear samples using size-exclusion high-performance liquid chromatography (SE-HPLC).
  • Quantified protein levels using enzyme-linked immunosorbent assays and kinetic assays.

Related Experiment Videos

Main Results:

  • Nine out of 13 proteins were significantly higher in nonstimulated tears.
  • Immunoglobulins (IgM, IgA) decreased with increasing tear flow.
  • Serum-derived proteins (IgG, albumin, transferrin) showed an initial drop, then stabilized.

Conclusions:

  • Tear protein concentration is highly sensitive to stimulation, with distinct patterns for different protein types.
  • Noninvasive tear collection is effective for studying these dynamic changes.
  • Characterizing tear composition requires more than just differentiating between stimulated and nonstimulated states.