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Published on: April 5, 2019
Immunoassay for human serum hemojuvelin
Carole Brasse-Lagnel1, Maura Poli, Céline Lesueur
1Laboratoire de Biochimie Médicale, Hôpital Universitaire de Rouen, EA4309 Endothélium Microvasculaire et Lésions Cérébrales Néonatales, Université de Rouen, Rouen, France. carole.lagnel@univ-rouen.fr
Haematologica
|August 18, 2010
Summary
A new enzyme-linked immunosorbent assay quantifies soluble hemojuvelin in serum. This assay reveals lower hemojuvelin in TMPRSS6-mutated iron deficiency anemia and higher levels in anemia of chronic disease.
Area of Science:
- Biochemistry
- Molecular Biology
- Hematology
Background:
- Hemojuvelin regulates hepcidin expression and iron homeostasis.
- Soluble hemojuvelin is produced via furin or matriptase 2 (TMPRSS6) cleavage.
- Serum hemojuvelin levels are implicated in iron deficiency and hypoxia responses.
Purpose of the Study:
- Develop and validate the first enzyme-linked immunosorbent assay (ELISA) for human serum hemojuvelin.
- Quantify soluble hemojuvelin levels in various patient populations.
Main Methods:
- Utilized a recombinant human repulsive guidance molecule-c peptide and a polyclonal anti-hemojuvelin antibody.
- Employed immunoprecipitation to detect native soluble hemojuvelin.
- Validated the ELISA for robustness and accuracy.
Main Results:
- The validated ELISA demonstrated high robustness with low intra- and inter-coefficients of variance (2.6%–15%).
- Quantified hemojuvelin levels in controls between 0.88–1.14 mg/L.
- Observed significantly lower serum hemojuvelin in patients with TMPRSS6 mutations (iron-refractory iron-deficiency anemia) and higher levels in patients with anemia of chronic disease compared to controls.
Conclusions:
- The developed ELISA is specific and sensitive for quantifying soluble hemojuvelin in human serum.
- This assay is a valuable tool for investigating hemojuvelin's physiological roles.
- Findings suggest hemojuvelin levels are altered in specific anemia types.
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