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Related Concept Videos

Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...

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Related Experiment Video

Updated: Jun 10, 2026

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

Clb2 and the APC/C(Cdh1) regulate Swe1 stability.

Kobi J Simpson-Lavy1, Michael Brandeis

  • 1The Department of Genetics, The Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.

Cell Cycle (Georgetown, Tex.)
|August 18, 2010
PubMed
Summary
This summary is machine-generated.

Swe1/Wee1 controls mitotic entry and stress-induced G2 arrest. Its degradation is regulated by Clb2-Cdk1, Hsl1, and Cdc5, with Clb2 interaction crucial for Swe1 accumulation.

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CRISPR-Mediated Reorganization of Chromatin Loop Structure
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CRISPR-Mediated Reorganization of Chromatin Loop Structure

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Related Experiment Videos

Last Updated: Jun 10, 2026

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
10:50

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Published on: March 14, 2019

CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

Area of Science:

  • Cell cycle regulation
  • Molecular biology
  • Yeast genetics

Background:

  • Swe1/Wee1 is a key regulator of mitotic entry, inhibiting Clb2-Cdk1.
  • Swe1 accumulation contributes to stress-induced G2 arrest.
  • APC/C(Cdh1) targets Cdc5, Clb2, and Hsl1 for degradation, influencing Swe1 stability.

Purpose of the Study:

  • To investigate the regulatory mechanisms controlling Swe1 degradation.
  • To elucidate the roles of Clb2, Hsl1, and Cdc5 in Swe1 stability.
  • To understand how replication fork stress affects Swe1 accumulation.

Main Methods:

  • Genetic analysis of Saccharomyces cerevisiae mutants (e.g., clb2Delta, cdh1Delta).
  • Expression of non-degradable mutant proteins (Cdc5(T29A), Hsl1(mdb/mkb), Clb2(ME)).
  • Induction of replication fork stress using hydroxyurea.

Main Results:

  • The clb2Deltacdh1Delta double mutant showed no detectable Swe1, indicating constitutive degradation.
  • Cdh1 inactivation stabilized Cdc5 and Hsl1, preventing Swe1 accumulation.
  • Non-degradable Hsl1 prevented Swe1 accumulation even in wild-type Clb2 cells.
  • Swe1 accumulation was restored in mutants upon hydroxyurea treatment.
  • Swe1 requires Clb2 interaction to accumulate, as shown by the Clb2(ME) mutant.

Conclusions:

  • Clb2-Cdk1 likely protects Swe1 from degradation.
  • Hsl1 mediates de-protection, enabling Cdc5-dependent degradation.
  • Swe1 levels are tightly regulated by monitoring Clb2, Hsl1, and Cdc5.