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Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

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Related Experiment Video

Updated: Jun 10, 2026

Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions
07:16

Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions

Published on: January 5, 2024

On-chip immunoprecipitation for protein purification.

Mairi E Sandison1, Sarah A Cumming, Walter Kolch

  • 1Integrative and Systems Biology, FBLS, University of Glasgow, Glasgow, G12 8QQ, UK. m.sandison@bio.gla.ac.uk

Lab on a Chip
|August 18, 2010
PubMed
Summary

This study introduces a miniaturized immunoprecipitation (µIP) technique for efficient on-chip protein purification. The optimized method successfully isolates affinity-tagged proteins from complex bacterial mixtures.

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

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Last Updated: Jun 10, 2026

Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions
07:16

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Published on: January 5, 2024

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Immunoprecipitation (IP) is a standard technique for protein purification.
  • Existing IP methods can be time-consuming and require large sample volumes.
  • There is a need for more efficient and miniaturized protein purification strategies.

Purpose of the Study:

  • To develop and optimize a miniaturized, polymer-supported immunoprecipitation (µIP) method.
  • To enable on-chip purification of proteins from complex biological mixtures.
  • To demonstrate the efficacy of µIP for isolating specific proteins.

Main Methods:

  • Fabrication of a 4 µl polydimethylsiloxane (PDMS) column.
  • Functionalization of the column with covalently bound antibodies.
  • Optimization of antibody immobilization, blocking, sample incubation, and washing steps.

Main Results:

  • Successfully optimized all critical aspects of the µIP protocol.
  • Demonstrated the µIP method's ability to purify proteins on-chip.
  • Obtained purified fractions of affinity-tagged protein from a bacterial lysate.

Conclusions:

  • The developed µIP method offers a miniaturized and efficient approach for protein purification.
  • This on-chip technique is suitable for processing complex biological samples.
  • µIP provides a valuable tool for protein isolation in various biochemical and biotechnological applications.