Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uninterrupted optical resolution of identical point scatterers undergoing nanometric changes in distance.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Phenoxazines with a Phototransferable <i>N</i>-Acetyl Group and Acrylate Linker: Assembly by C-H Activation, Photoconversion to Fluorescent Dyes, Biolabeling, and Super-Resolution Imaging.

Journal of the American Chemical Society·2026
Same author

Robust carotid artery perfusion protocol for turtle brain fixation enabling multiscale imaging from magnetic resonance to super-resolution microscopy.

Methods (San Diego, Calif.)·2026
Same author

Synthesis of Benzo[<i>b</i>]thiophene 1,1-Dioxides via Pd-Catalyzed Sulfinylation of Aryl Triflates and Their Use as Large Stokes Shift Fluorophores for Multicolor Live-Cell Imaging with Self-Labeling Tags.

JACS Au·2026
Same author

Photoactivatable Carborhodol and Carborhodamine Dyes with One Cleavable Group: Synthesis, Spectra, and Fluorescence Nanoscopy Applications.

JACS Au·2026
Same author

Glycosaminoglycan-functionalized hydrogels for sustained delivery of tissue inhibitor of metalloproteinase-3 mediating matrix metalloprotease inhibition and extracellular matrix stabilization.

Bioactive materials·2026

Related Experiment Video

Updated: Jun 10, 2026

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

Dynamic imaging of colloidal-crystal nanostructures at 200 frames per second.

Marcel A Lauterbach1, Chaitanya K Ullal, Volker Westphal

  • 1Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37075 Göttingen, Germany.

Langmuir : the ACS Journal of Surfaces and Colloids
|August 19, 2010
PubMed
Summary

Fast Stimulated Emission Depletion (STED) microscopy overcomes light microscopy limits, enabling dynamic imaging of 200 nm colloidal crystal formation. This technique visualizes 30 nm voids and defect annealing with 5 ms temporal resolution.

More Related Videos

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
10:56

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Published on: May 20, 2014

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
10:18

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

Related Experiment Videos

Last Updated: Jun 10, 2026

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
10:56

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Published on: May 20, 2014

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
10:18

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

Area of Science:

  • Colloidal science
  • Optical microscopy
  • Materials science

Background:

  • Conventional light microscopy, including confocal microscopy, is limited by diffraction, hindering dynamic, noninvasive imaging of nanoscale colloidal structures.
  • Resolving fine details and dynamic processes in colloidal systems requires advanced imaging techniques beyond traditional light microscopy's capabilities.

Purpose of the Study:

  • To demonstrate the capability of Fast Stimulated Emission Depletion (STED) microscopy for high-resolution, dynamic imaging of colloidal nanostructures.
  • To resolve the formation process of a colloidal crystal, including small voids and defect dynamics, in real-time.

Main Methods:

  • Utilizing Fast Stimulated Emission Depletion (STED) microscopy for enhanced spatial and temporal resolution.
  • Imaging the self-assembly of 200 nm colloidal particles into a monolayer crystal structure.

Main Results:

  • Achieved resolution of colloidal crystal formation with voids as small as 30 nm.
  • Demonstrated real-time visualization of the annealing of point defects during crystal formation with a temporal resolution of 5 ms.
  • Successfully imaged dynamic processes in colloidal systems previously precluded by diffraction limits.

Conclusions:

  • Fast STED microscopy offers a powerful tool for the dynamic, noninvasive imaging of colloidal nanostructures at the nanoscale.
  • The technique enables the study of crystal formation dynamics, including defect evolution, providing insights into self-assembly processes.