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Activation of RASSF2A by p300 induces late apoptosis through histone hyperacetylation

Chungang Liu1, Yunhui Pan, Xiuli Wang

  • 1School of Life Science, Northeast Normal University, Changchun 130024, Peoples Republic of China.

Cell Biology International
|August 19, 2010
PubMed

Insights

p300 protein induces late apoptosis by increasing Ras-associated family 2A (RASSF2A) expression in gastric cancer cells. This occurs via Sp1 transcription factor cooperation and histone acetylation at the RASSF2A promoter.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Ras-associated family 2A (RASSF2A) and p300 are involved in apoptosis.
  • The precise relationship between RASSF2A and p300 in apoptosis induction is not well understood.

Purpose of the Study:

  • To investigate the interrelationship between p300 and RASSF2A in the induction of apoptosis.
  • To elucidate the molecular mechanisms by which p300 influences RASSF2A expression and subsequent apoptosis.

Main Methods:

  • Cell culture of human gastric cancer SGC-7901 (p53-mutant) and 293T cells.
  • Analysis of RASSF2A expression and apoptosis induction.
  • Chromatin immunoprecipitation (ChIP) assays to assess histone acetylation and protein binding.
  • Investigation of the role of transcription factor Sp1.

Main Results:

  • p300 induces late apoptosis through up-regulation of RASSF2A in SGC-7901 cells.
  • p300 stimulates RASSF2A expression in 293T cells, cooperating with Sp1.
  • p300 induces histone H3 and H4 hyperacetylation at the RASSF2A promoter.
  • p300 and Sp1 exhibit reciprocal facilitation of binding to the RASSF2A promoter.

Conclusions:

  • Sp1-mediated RASSF2A gene transcription is activated by p300 via histone acetylation.
  • This p300-mediated activation of RASSF2A plays a significant role in inducing late apoptosis.

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