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Published on: March 30, 2019

Down-regulation of ING4 is associated with initiation and progression of lung cancer

Qiu-shi Wang1, Ming Li, Lin-you Zhang

  • 1Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Histopathology
|August 19, 2010
PubMed
Abstract

Insights

Reduced ING4 expression, particularly in the nucleus, is observed in lung cancers and correlates with advanced tumour grade and metastasis. This suggests ING4 analysis could aid in lung cancer diagnosis and prognosis.

Area of Science:

  • Oncology
  • Molecular Biology

Background:

  • ING4 is a tumour suppressor gene involved in cell cycle regulation and apoptosis.
  • ING4 expression is frequently inhibited in various cancers, including gliomas and breast tumours.

Purpose of the Study:

  • To investigate the expression levels of ING4 in lung cancer tissues.
  • To determine the correlation between ING4 expression and clinicopathological features of lung cancer.

Main Methods:

  • Immunohistochemistry was performed on 246 lung tumour tissues.
  • Quantitative reverse transcriptase-polymerase chain reaction and Western blotting were used to assess ING4 mRNA and protein levels.

Main Results:

  • Reduced ING4 nuclear and cytoplasmic expression was found in lung cancer tissues compared to normal tissues.
  • Lower ING4 expression correlated with higher tumour grade (grade III vs. grades I-II), advanced tumour stage, and lymph node metastasis.
  • A higher proportion of tumour cells showed cytoplasmic rather than nuclear ING4 expression.

Conclusions:

  • Overall inhibition of ING4 expression and a shift towards cytoplasmic localization may play a role in lung cancer initiation and progression.
  • ING4 expression analysis may serve as a valuable clinical diagnostic and prognostic biomarker for lung cancer.

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