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Related Experiment Video

Updated: Jun 10, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

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Published on: January 9, 2017

Focused laser beam effects in optical particle sizing by dynamic light scattering.

D A Ross

    Applied Optics
    |August 19, 2010
    PubMed
    Summary

    Focused laser beams distort dynamic light scattering particle sizing. Backscatter measurements are preferred for accurate particle-size distribution, as they most closely resemble plane wave results.

    Area of Science:

    • Optical Physics
    • Particle Characterization

    Background:

    • Dynamic light scattering (DLS) is a standard technique for measuring particle size.
    • The accuracy of DLS depends on the properties of the incident laser beam and scattering geometry.
    • Focused laser beams, commonly used in DLS, may introduce distortions not present with plane waves.

    Purpose of the Study:

    • To analyze the impact of a focused laser beam on optical particle sizing using dynamic light scattering.
    • To compare the autocorrelation functions obtained with focused beams versus plane waves.
    • To determine the optimal scattering conditions for accurate particle-size distribution analysis with focused beams.

    Main Methods:

    • Numerical integration was used to calculate the first-order electric-field autocorrelation function for a focused beam.

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  • Asymptotic expansion was employed to approximate the autocorrelation function.
  • The results were compared with the theoretical autocorrelation function for a plane wave.

    • Main Results:

    • The autocorrelation function obtained with a focused laser beam is distorted compared to the plane wave autocorrelation.
    • This distortion is most pronounced at forward-scattering angles.
    • While the focused beam autocorrelation can be modeled, it introduces inaccuracies.

    Conclusions:

    • Focused laser beams significantly affect dynamic light scattering measurements.
    • For accurate particle-size distribution, especially when inverting correlation data, backscattering is preferred.
    • Backscatter measurements with focused beams most closely approximate the results obtained with plane waves, ensuring higher accuracy and resolution.