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Related Experiment Video

Updated: Jun 10, 2026

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
06:32

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

GRIM-19 Expression and Function in Human Gliomas.

Yong-Hao Jin1, Shin Jung, Shu-Guang Jin

  • 1Department of Neurosurgery & Brain Tumor Research Laboratory, Chonnam National University Hwasun Hospital & Medical School, Gwangju, Korea.

Journal of Korean Neurosurgical Society
|August 19, 2010
PubMed
Summary
This summary is machine-generated.

GRIM-19 expression correlates with glioma grade and type, potentially regulating cell death. Overexpression of GRIM-19 in glioblastoma cells enhances sensitivity to combined therapies and induces apoptosis.

Keywords:
Cell lineGRIM-19Gene FishingGlioblastomaHuman glioma

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Last Updated: Jun 10, 2026

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
06:32

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Published on: January 9, 2019

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12:52

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Published on: November 28, 2015

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09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • Gliomas are primary brain tumors with diverse pathological types and grades.
  • Understanding gene expression patterns is crucial for diagnosing and treating gliomas.
  • GRIM-19's role in glioma pathogenesis requires further investigation.

Purpose of the Study:

  • To investigate the correlation between GRIM-19 expression and glioma subtypes and grades.
  • To elucidate the functional role of GRIM-19 in human gliomas.

Main Methods:

  • RNA differential display and RT-PCR were used to analyze GRIM-19 expression in various glioma tissues.
  • Functional studies involved transfecting a human glioblastoma cell line (U343MG-A) with GRIM-19.
  • Cell morphology, migration, invasion, and proliferation were assessed using microscopy, Matrigel assay, and MTT assay.

Main Results:

  • GRIM-19 expression was higher in astrocytic tumors than oligodendroglial tumors and increased with tumor grade, with glioblastomas showing the highest levels.
  • Transfection of GRIM-19 altered glioblastoma cell morphology, making them larger and more spindly.
  • GRIM-19 overexpression increased glioblastoma cell sensitivity to interferon-beta and retinoic acid, linked to apoptosis.

Conclusions:

  • GRIM-19 expression is associated with glioma progression and pathological type.
  • GRIM-19 may function as a regulator of apoptosis in human gliomas.
  • GRIM-19 could be a potential therapeutic target in glioma treatment.