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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

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Expression of micro-RNA-145 is regulated by a highly conserved genomic sequence 3' to the pre-miR

Gaspare La Rocca1, Bin Shi, Laura Sepp-Lorenzino

  • 1Department of Cancer Biology, Thomas Jefferson University and Kimmel Cancer Center, Philadelphia, Pennsylvania 19107, USA.

Journal of Cellular Physiology
|August 19, 2010
PubMed

Insights

Micro-RNA-145 (miR145) suppresses tumor growth and aids stem cell differentiation. Its expression is modulated by flanking genomic sequences, with a conserved 3' region being crucial.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Micro-RNA-145 (miR145) is a known tumor suppressor microRNA.
  • miR145 significantly inhibits cancer cell growth and is involved in human stem cell differentiation.

Purpose of the Study:

  • To isolate and characterize the human genomic sequence of pre-miR145 and its flanking regions.
  • To investigate the role of flanking sequences in regulating miR145 expression.

Main Methods:

  • Isolation of an 864 bp human genomic sequence containing pre-miR145 and flanking regions.
  • Transfection of cloned genomic sequences into cells to assess miR145 expression.
  • Analysis of sequence conservation in flanking regions.

Main Results:

  • The cloned miR145 genomic sequence successfully expressed mature miR145 in transfected cells.
  • Flanking sequences on both sides of the pre-miR sequence were shown to modulate expression levels.
  • A highly conserved sequence located 3' to the pre-miR sequence was identified as critical for miR145 expression.

Conclusions:

  • The human miR145 gene locus contains regulatory elements within its flanking sequences.
  • A conserved 3' sequence plays a vital role in controlling miR145 expression, highlighting its importance in biological regulation.

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