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Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging

Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging

Published on: September 12, 2025

Multiphoton microscopic imaging of normal human rectum tissue.

Nenrong Liu¹, Gang Chen, Jianxin Chen

¹Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.

|August 19, 2010

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Multiphoton microscopy (MPM) visualizes human rectal tissue microstructures. This advanced imaging shows potential for early rectal cancer diagnosis by analyzing tissue components.

Area of Science:

Biomedical Imaging
Gastroenterology
Optical Microscopy

Background:

Early detection of gastrointestinal pathologies, particularly rectal cancer, is crucial for effective treatment.
Understanding the microstructure of rectal mucosa and submucosa is key to identifying pathological changes.
Multiphoton microscopy (MPM) offers advanced capabilities for non-invasive tissue imaging.

Purpose of the Study:

To investigate the potential of multiphoton microscopy (MPM) for imaging human rectal mucosa and submucosa.
To assess the capability of MPM in visualizing key tissue microstructures and their variations.
To explore the application of MPM in the clinical diagnosis and monitoring of early rectal cancer.

Main Methods:

Utilized multiphoton microscopy (MPM) based on two-photon excited fluorescence and second harmonic generation signals.

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Last Updated: Jun 10, 2026

Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging

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Published on: September 12, 2025

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Imaged microstructures of human rectal mucosa and submucosa.

Analyzed morphology and distribution of goblet cells, intestinal glands, collagen, elastic fibers, and blood vessels.

Main Results:

Clearly monitored morphology and distribution of goblet cells, intestinal glands, and collagen fibers in the mucosa.
Distinctly obtained content and distribution of collagen, elastic fibers, and blood vessels in the submucosa.
Observed variations in tissue components relevant to gastrointestinal pathology, including early rectal cancer.

Conclusions:

MPM successfully visualized microstructural components of human rectal mucosa and submucosa.
The technique demonstrated the ability to detect variations in tissue composition relevant to rectal cancer.
MPM shows significant potential for in vivo clinical diagnosis and monitoring of early rectal cancer.