Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Superoxide generation by human monocytes and macrophages.

S J Weiss, G W King, A F LoBuglio

    American Journal of Hematology
    |January 1, 1978
    PubMed
    Summary

    Human monocytes and macrophages generate significant superoxide during phagocytosis. This superoxide production is crucial for bactericidal functions and other cellular activities.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Characterization of intentional lurasidone ingestions using the United States National Poison Data System.

    Clinical toxicology (Philadelphia, Pa.)·2020
    Same author

    Using motion capture technology to measure the effects of magnification loupes on dental operator posture: A pilot study.

    Work (Reading, Mass.)·2018
    Same author

    Language disparities in patients transported by emergency medical services.

    The American journal of emergency medicine·2015
    Same author

    Modification of a stimulus-reinforcer interaction by blocking.

    Behavioural processes·2014
    Same author

    Extracellular matrix determinants and the regulation of cancer cell invasion stratagems.

    Journal of microscopy·2013
    Same author

    Assessing natural and disturbed population structure in European grayling Thymallus thymallus: melding phylogeographic, population genetic and jurisdictional perspectives for conservation planning.

    Journal of fish biology·2013

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Superoxide (O2.-) is a reactive oxygen species with critical roles in cellular signaling and defense.
    • Human monocytes and macrophages are key immune cells involved in phagocytosis and pathogen clearance.

    Purpose of the Study:

    • To quantify intracellular and extracellular superoxide generation in human monocytes and macrophages.
    • To investigate the role of superoxide production during phagocytosis of zymosan particles.
    • To assess superoxide generation in monocytes from patients with chronic granulomatous disease.

    Main Methods:

    • Nitroblue tetrazolium (NBT) reduction assay was used to measure superoxide production.
    • Superoxide dismutase (SOD) was employed to confirm superoxide-specific generation.
    • Phagocytosis was induced using zymosan particles.

    Main Results:

    • Resting human monocytes produced 1.8 +/- 0.9 nmoles of superoxide per 10(6) cells.
    • Superoxide generation increased significantly to 16.8 +/- 2.8 nmoles/10(6) cells during zymosan phagocytosis.
    • Human macrophages exhibited comparable superoxide production levels.
    • Monocytes from a patient with chronic granulomatous disease showed no increase in superoxide production during phagocytosis.

    Conclusions:

    • Human monocytes and macrophages are capable of substantial superoxide generation during phagocytosis.
    • This superoxide production likely plays a vital role in the bactericidal activity of these immune cells.
    • Defective superoxide production, as seen in chronic granulomatous disease, impairs phagocytic defense mechanisms.

    Related Experiment Videos