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Expression of herpesvirus in adherent cells derived from bone marrow of latently infected guinea pigs
Abstract:
The role of bone marrow adherent cells in the latency of guinea pig herpes-like virus (GPHLV) was explored. Cultures of macrophage-enriched adherent cells derived from infected guinea pigs were examined for evidence of latent GPHLV infection. Expression of the virus was detected in these cultures 9 to 10 days after in vitro cultivation. Increasing virus infectivity titers as well as light and electron microscopic evidence of virion assembly in macrophages and fibroblasts were demonstrated. Infections virus was detected in the bone marrow adherent cells that had attached for 30 or 120 minutes but only following reverse cocultivation. The data showed not only that the bone marrow adherent cells were susceptible to GPHLV in vitro but also that GPHLV was harbored by the macrophage-enriched bone marrow population in vivo in latently infected guinea pigs.
Insights
Bone marrow adherent cells, particularly macrophages, harbor latent guinea pig herpes-like virus (GPHLV). These cells showed GPHLV expression and virion assembly during in vitro cultivation, confirming their role in viral latency.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- The guinea pig herpes-like virus (GPHLV) is a significant pathogen in guinea pigs.
- Understanding viral latency mechanisms is crucial for developing effective treatments and control strategies.
Purpose of the Study:
- To investigate the role of bone marrow adherent cells in the latency of GPHLV.
- To determine if macrophage-enriched bone marrow populations harbor latent GPHLV in vivo.
Main Methods:
- Culturing macrophage-enriched bone marrow adherent cells from GPHLV-infected guinea pigs.
- Assessing GPHLV expression and infectivity via in vitro cultivation and reverse co-cultivation.
- Utilizing light and electron microscopy to visualize virion assembly.
Main Results:
- GPHLV expression was detected in bone marrow adherent cell cultures 9-10 days post-cultivation.
- Increasing virus infectivity titers and evidence of virion assembly in macrophages and fibroblasts were observed.
- GPHLV was detected in bone marrow adherent cells following reverse co-cultivation, indicating latent infection.
Conclusions:
- Bone marrow adherent cells are susceptible to GPHLV infection in vitro.
- Macrophage-enriched bone marrow populations harbor latent GPHLV in vivo.
- These findings highlight the critical role of bone marrow cells in GPHLV persistence.