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Related Concept Videos

Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Role of Microtubules in Cell Wall Deposition01:02

Role of Microtubules in Cell Wall Deposition

Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of disassembly and...
The Phragmoplast01:59

The Phragmoplast

Cell division is essential for organismal growth and development. In animal cells, the central spindle and its associated proteins form the midbody, a structure that has an essential role in cytokinesis. In plants, the central spindle, along with the microtubules, actin, and other cell components, matures into the phragmoplast, which is necessary for cytokinesis. Unlike the stationary midbody, the phragmoplast expands centrifugally, eventually leading to the formation of the new cell wall.
The...
The Phragmoplast01:59

The Phragmoplast

Cell division is essential for organismal growth and development. In animal cells, the central spindle and its associated proteins form the midbody, a structure that has an essential role in cytokinesis. In plants, the central spindle, along with the microtubules, actin, and other cell components, matures into the phragmoplast, which is necessary for cytokinesis. Unlike the stationary midbody, the phragmoplast expands centrifugally, eventually leading to the formation of the new cell wall.
The...
Microtubule Formation01:23

Microtubule Formation

Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation of...

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Related Experiment Video

Updated: Jun 10, 2026

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
06:31

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

Published on: December 12, 2015

Microtubule dynamics in plant cells.

Henrik Buschmann1, Adrian Sambade, Edouard Pesquet

  • 1Department of Cell and Developmental Biology, John Innes Centre, Norwich NR47UH, United Kingdom.

Methods in Cell Biology
|August 20, 2010
PubMed
Summary
This summary is machine-generated.

Studying plant cell microtubule dynamics requires careful choices in species, probes, and imaging techniques. New methods improve long-term visualization by addressing limitations in live-cell imaging and photobleaching.

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High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
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High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

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Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
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Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

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Related Experiment Videos

Last Updated: Jun 10, 2026

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
06:31

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

Published on: December 12, 2015

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
10:23

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

Published on: April 20, 2017

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
07:52

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

Published on: May 23, 2020

Area of Science:

  • Plant Cell Biology
  • Cytoskeleton Dynamics
  • Microscopy Techniques

Background:

  • Studying microtubule dynamics in plants presents unique challenges due to cell size, lack of centrosomes, and the need for transgenic models.
  • Existing fluorescent probes for microtubules have limitations, including potential overstabilization and bundling, and a scarcity of minus-end markers.

Purpose of the Study:

  • To outline critical considerations and compromises in studying plant microtubule dynamics.
  • To present solutions for overcoming key technical hurdles in live-cell imaging of plant microtubules.

Main Methods:

  • Utilizing transgenic plants (Arabidopsis, tobacco BY-2) for microtubule visualization.
  • Employing fluorescence-tagged microtubule proteins and developing novel biochambers for extended live-cell imaging.
  • Implementing advanced imaging strategies to mitigate photobleaching during long-term observations.

Main Results:

  • Demonstrated the utility of specific species and probes, while acknowledging their limitations.
  • Introduced a biochamber enabling sustained plant cell growth and development under microscopy with controlled gas exchange and reduced evaporation.
  • Described imaging strategies that effectively reduce photobleaching, facilitating long-term studies of microtubule dynamics.

Conclusions:

  • Careful selection of species, probes, and imaging parameters is crucial for accurate plant microtubule studies.
  • The developed biochamber and imaging strategies significantly enhance the feasibility of long-term, high-resolution visualization of plant microtubule dynamics.
  • These advancements provide a foundation for deeper investigation into plant-specific microtubule arrays and cellular processes.