A multiple-function stationary phase based on perhydro-26-membered hexaazamacrocycle for high-performance liquid...

Area of Science:

• Analytical Chemistry
• Materials Science

Background:

• Development of advanced stationary phases is crucial for improving chromatographic separations.
• Multimode stationary phases offer versatile retention mechanisms for complex sample analysis.

Purpose of the Study:

• To synthesize and characterize a novel hexaazamacrocycle-based silica stationary phase for high-performance liquid chromatography (HPLC).
• To evaluate the chromatographic performance and retention mechanisms of the new phase in both reversed-phase (RP) and normal-phase (NP) modes.

Main Methods:

• Synthesis of the L(1)GlySil stationary phase using 3-glycidoxypropyltrimethoxysilane.
• Characterization using infrared spectroscopy, elemental analysis, and thermogravimetric analysis.
• Chromatographic evaluation with various solute probes including aromatic compounds, pesticides, and phenols.

Main Results:

• The L(1)GlySil phase exhibited excellent chromatographic properties as a multimode stationary phase.
• The phase demonstrated multiple interaction sites: hydrophobic, hydrogen bonding, pi-pi, dipole-dipole, and acid-base equilibrium.
• Effective separation of aromatic compounds, organophosphorus pesticides, carbamate pesticides, and phenols was achieved.

Conclusions:

• The developed hexaazamacrocycle-silica phase is a versatile multimode stationary phase for HPLC.
• Its unique structure facilitates separation through diverse retention mechanisms.
• This new phase shows significant potential for analyzing various chemical compounds, including pesticides and phenols.