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Updated: Jun 10, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Application of a novel method for optimization of bioemulsan production in a miniaturized bioreactor
Gh Amoabediny1, M Rezvani, H Rashedi
1Department of Chemical Engineering, School of Engineering, University of Tehran, P.O. Box 11365-4563, Tehran, Iran. amoabediny@ut.ac.ir
Abstract:
A novel and economical method was used to optimize bacterial growth and bioemulsan production. Acinetobacter calcoaceticus PTCC 1641 was applied in an innovated synthetic medium comprised whey and mineral salt medium (MSM) in ratio of 1:1 in a shaken flask bioreactor. The optimum inoculum size, shaking frequency rate and filling volume (V(L)) using ventilation flasks were investigated that resulted in 5 vol.%, 300 rpm and 15 ml), respectively. The surface tension decreased around 48% during bioemulsan production. Due to determine bioemulsan properties, the reliability of blood agar method was confirmed as well.
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