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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: Jun 10, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

PmiRKB: a plant microRNA knowledge base.

Yijun Meng1, Lingfeng Gou, Dijun Chen

  • 1Department of Bioinformatics, State Key Laboratory of Plant Physiology and Biochemistry and Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.

Nucleic Acids Research
|August 20, 2010
PubMed
Summary
This summary is machine-generated.

A new plant microRNA knowledge base (PmiRKB) organizes miRNA data, including single nucleotide polymorphisms (SNPs) and target interactions. This resource aids in understanding plant gene regulation by microRNAs (miRNAs).

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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

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Last Updated: Jun 10, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Area of Science:

  • Plant molecular biology
  • Genomics
  • Bioinformatics

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression in plants.
  • Existing miRNA databases require updates to incorporate new datasets.
  • Comprehensive analysis of plant miRNA functions necessitates integrated data resources.

Purpose of the Study:

  • To develop a centralized knowledge base for plant microRNAs (PmiRKB).
  • To facilitate the organization and analysis of diverse plant miRNA-related datasets.
  • To provide tools for investigating miRNA-SNP interactions, transcriptional contexts, target validation, and precursor metabolism.

Main Methods:

  • Construction of the Plant microRNA Knowledge Base (PmiRKB) with four functional modules.
  • Inclusion of single nucleotide polymorphism (SNP) data for Arabidopsis and rice.
  • Integration of massively parallel signature sequencing and parallel analysis of RNA end (PARE) data.

Main Results:

  • The PmiRKB integrates SNP data to analyze effects on pre-miRNA structures and miRNA-target interactions.
  • The 'Pri-miR' module explores tissue-specific transcription of pre- and pri-miRNAs.
  • The 'MiR-Tar' and 'Self-reg' modules utilize PARE data for miRNA-target validation and precursor metabolism studies.

Conclusions:

  • PmiRKB offers a valuable resource for plant miRNA research.
  • The integrated data and functional modules enhance the understanding of miRNA-mediated gene regulation.
  • PmiRKB is freely accessible for scientific community use.