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Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Related Experiment Video

Updated: Jun 10, 2026

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
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Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

Microstructure characterization by angle-resolved scatter and comparison to measurements made by other techniques.

R D Jacobson, S R Wilson, G A Al-Jumaily

    Applied Optics
    |August 20, 2010
    PubMed
    Summary

    Angle-resolved scatter measurements provide accurate rms roughness values for various materials. This technique offers a valuable alternative to traditional scatter and profiling instruments for surface characterization.

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    Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction

    Published on: April 1, 2017

    Area of Science:

    • Surface metrology
    • Optical physics
    • Materials science

    Background:

    • Characterizing surface roughness is crucial for optical and mechanical applications.
    • Traditional methods like total integrated scatter and profilers have limitations.
    • Understanding scatter phenomena is key to precise surface analysis.

    Purpose of the Study:

    • To describe the theory and measurement of angle-resolved scatter (ARS).
    • To compare ARS-derived root-mean-square (rms) roughness values with other instruments.
    • To analyze the impact of instrument differences on roughness measurements.

    Main Methods:

    • Utilizing angle-resolved scatter (ARS) for surface roughness measurement.
    • Comparing ARS data with results from total integrated scatter (TIS) instruments.
    • Cross-validating with optical and mechanical profilers.
    • Analyzing differences in spatial frequency bandwidths and modulation transfer functions (MTFs).

    Main Results:

    • ARS measurements yielded comparable rms roughness values for four distinct materials.
    • Discrepancies between ARS and other methods were observed and analyzed.
    • Differences in spatial frequency bandwidth and MTF influenced measurement outcomes.
    • ARS demonstrated effectiveness in characterizing surface topography.

    Conclusions:

    • Angle-resolved scatter is a viable technique for accurate rms roughness determination.
    • Instrument-specific characteristics significantly impact surface metrology results.
    • ARS offers complementary information to traditional surface characterization tools.
    • Further investigation into instrument transfer functions is warranted for precise comparison.