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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Related Experiment Video

Updated: Jun 10, 2026

Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis
10:43

Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

Published on: November 6, 2019

Nonimaging light concentration using total internal reflection films.

G Ouellette, C E Waltham, R M Drees

    Applied Optics
    |August 20, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed nonimaging light concentrators using total internal reflection film. The prototype

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    Area of Science:

    • Optics and Photonics
    • Materials Science

    Background:

    • Nonimaging optics plays a crucial role in various applications, including solar energy and illumination.
    • Traditional light concentrators often rely on specular reflecting materials, which can be complex to fabricate.

    Purpose of the Study:

    • To introduce a novel method for fabricating nonimaging light concentrators using total internal reflection (TIR) film.
    • To evaluate the performance of a prototype TIR-based concentrator.

    Main Methods:

    • Fabrication of a nonimaging light concentrator prototype utilizing TIR film.
    • Experimental testing and performance evaluation of the prototype.
    • Comparison of experimental results with predictions from ray-tracing simulations.

    Main Results:

    • The fabricated prototype demonstrated performance consistent with ray-tracing code predictions.
    • The TIR film concentrator achieved performance comparable to concentrators made from traditional specular reflecting materials.

    Conclusions:

    • Total internal reflection film offers a viable and effective alternative for fabricating nonimaging light concentrators.
    • This method presents a promising approach for developing efficient and potentially cost-effective light concentration solutions.