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Updated: Jun 10, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Iterative method for zero-order suppression in off-axis digital holography
Nicolas Pavillon1, Cristian Arfire, Isabelle Bergoënd
1Advanced Photonics Laboratory, Microvision and Microdiagnostics Group (MVD), Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
Abstract:
We propose a method to suppress the so-called zero-order term in a hologram, based on an iterative principle. During the hologram acquisition process, the encoded information includes the intensities of the two beams creating the interference pattern, which do not contain information about the recorded complex wavefront, and that can disrupt the reconstructed signal. The proposed method selectively suppresses the zero-order term by employing the information obtained during wavefront reconstruction in an iterative procedure, thus enabling its suppression without any a priori knowledge about the object. The method is analyzed analytically and its convergence is studied. Then, its performance is shown experimentally. Its robustness is assessed by applying the procedure on various types of holograms, such as topographic images of microscopic specimens or speckle holograms.
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