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Updated: Jun 10, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

High-resolution methods for fluorescence retrieval from space.

Marina Mazzoni¹, Pierluigi Falorni, Wouter Verhoef

¹Istituto di Fisica Applicata Nello Carrara del Consiglio Nazionale delle Ricerche, via Madonna del Piano 10, 50019 Sesto Fiorentino, Italy. M.Mazzoni@ifac.cnr.it

|August 20, 2010

Summary

This summary is machine-generated.

This study demonstrates a method to accurately retrieve weak chlorophyll fluorescence signals from space using oxygen absorption bands. The developed algorithm achieves better than 10% accuracy for hyperspectral sensors.

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Area of Science:

Earth and Atmospheric Sciences
Remote Sensing
Spectroscopy

Background:

Retrieving weak fluorescence signals from space is crucial for monitoring vegetation health.
Oxygen absorption bands (O2A and O2B) offer potential windows for such measurements.
Accurate retrieval requires accounting for atmospheric effects and sensor characteristics.

Purpose of the Study:

To develop and validate an algorithm for retrieving sun-induced chlorophyll fluorescence from space.
To assess the accuracy of fluorescence retrieval under realistic noise conditions.
To evaluate the algorithm's performance across various hyperspectral sensor resolutions.

Main Methods:

Simulated top-of-atmosphere radiance using a radiative-transfer program at high spectral resolution (0.1 cm⁻¹).
Modeled reflectance with cubic splines and fluorescence with Voigt functions.
Applied sensor radiance residual minimization with multiplicative noise for realistic simulations.

Main Results:

The developed algorithm demonstrated the capability to retrieve fluorescence with better than 10% accuracy.
Performance was evaluated for instrument resolutions from 0.4 cm⁻¹ to 2 cm⁻¹.
Successful fluorescence retrieval was achieved at OCO and TANSO-FTS instrument spectral resolutions within the O2A band.

Conclusions:

Accurate retrieval of sun-induced chlorophyll fluorescence from space is feasible using oxygen absorption bands.
The developed algorithm is robust and applicable to existing and future hyperspectral sensors.
This method advances remote sensing capabilities for vegetation monitoring.