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Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)
Published on: October 17, 2010
Triple-frequency symmetric subtraction scheme for nonresonant background suppression in coherent anti-Stokes Raman
Chao Fang1, Fake Lu, Wei Zheng
1Optical Bioimaging Laboratory, Department of Bioengineering, Faculty of Engineering, National University of Singapore, 117576 Singapore.
Optics Express
|August 20, 2010
Abstract:
We report a unique triple-frequency symmetric subtraction scheme to effectively remove the nonresonant background in coherent anti-Stokes Raman scattering (CARS) microscopy. Theoretical and experimental studies show that this unique scheme has an optimal performance for high contrast vibrational imaging, particularly useful when the resonant signal was larger than or comparable to the nonresonant background.
