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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Updated: Jun 10, 2026

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

Single molecule transcription profiling with AFM.

Jason Reed¹, Bud Mishra, Bede Pittenger

¹Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA 90095, USA.

|August 20, 2010

Summary

This summary is machine-generated.

This study introduces single molecule, ordered restriction mapping with atomic force microscopy (AFM) for sensitive gene expression profiling. This novel molecular nanotechnology approach enhances transcription level measurement from minute samples.

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Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

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Area of Science:

Molecular Biology
Nanotechnology
Genomics

Background:

Established gene expression profiling methods like microarrays have sensitivity limitations.
There is a growing need for sensitive techniques to analyze gene expression in minute samples, such as single cells.
Molecular nanotechnologies offer potential solutions for low-abundance sample analysis.

Purpose of the Study:

To investigate the use of single molecule, ordered restriction mapping combined with AFM for measuring gene transcription levels.
To analyze the mathematical framework and critical error sources for this technique.
To demonstrate the construction of high-density, single-molecule restriction maps.

Main Methods:

Utilizing single molecule, ordered restriction mapping.
Employing Atomic Force Microscopy (AFM) for high-resolution imaging.
Applying coding theory for mathematical modeling and error analysis.
Constructing restriction maps from plasmid and cDNA molecules using two enzymes.

Main Results:

Demonstrated the feasibility of constructing high-density, single-molecule, ordered restriction maps.
Successfully mapped restriction sites on both plasmid and cDNA molecules.
Identified and analyzed critical error sources relevant for future study design.

Conclusions:

Single molecule, ordered restriction mapping with AFM is a promising technique for sensitive gene expression profiling.
The mathematical analysis provides a guide for optimizing future experiments with low-abundance samples.
This method offers a novel approach for analyzing transcription levels in challenging biological samples.