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Published on: February 15, 2019
Activity-directed identification of maize kernel peroxidases associated with postharvest insect resistance
Robert Winkler1, Silverio García-Lara
1Department of Biotechnology and Food Engineering, Tecnológico de Monterrey, ITESM-Campus Monterrey, Eugenio Garza Sada 2501, CP 64849, Monterrey, NL, Mexico.
Abstract:
Maize grain peroxidases are associated with insect resistance. In this study we present an activity directed approach for the identification of peroxidases localized in different grain tissues. With drastically reduced proteomic effort we could identify different peroxidases for maize weevil susceptible and resistant genotypes of maize. Our strategy also allows for the identification of previously unknown plant peroxidases.
